NDLI: Análisis de la Variabilidad Genética entre treinta accesiones de tarwi (Lupinus mutabilis Sweet) usando marcadores moleculares ISSR

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Análisis de la Variabilidad Genética entre treinta accesiones de tarwi (Lupinus mutabilis Sweet) usando marcadores moleculares ISSR

Content Provider	Semantic Scholar
Author	Chirinos-Arias, Michelle C. Jiménez, Jorge Enrique Vilca-Machaca, Lizbeth S.
Copyright Year	2015
Abstract	Con el fin de realizar el analisis de variabilidad genetica inter-accesion de treinta accesiones de tarwi (L. mutabilis Sweet) pertenecientes al Banco de Germoplasma del Instituto Nacional de Innovacion Agraria (INIA). Se extrajo el ADN de 300 plantas, se construyeron bulks, se estandarizo el protocolo de amplificacion de los marcadores moleculares Inter Simple Sequence Repeat (ISSR), de los cuales se eligio a los mas polimorficos y nitidos para corrida en gel de acrilamida. Encontrandose 255 bandas con 8 iniciadores ISSR. El analisis de la variabilidad genetica con estos iniciadores comprobo una alta variabilidad genetica de las muestras en estudio. Observandose tambien un polimorfismo relativamente alto para una especie autogama como L. mutabilis. Finalmente los fenogramas mostraron una relacion con la ubicacion geografica, posiblemente debido al flujo genico in situ debido al intercambio o venta de semillas en ferias o mercados aledanos a la zona de colecta.
Starting Page	17
Ending Page	30
Page Count	14
File Format	PDF HTM / HTML
DOI	10.17268/sci.agropecu.2015.01.02
Alternate Webpage(s)	http://www.scielo.org.pe/pdf/agro/v6n1/a02v6n1.pdf
Alternate Webpage(s)	http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/download/808/736
Alternate Webpage(s)	https://doi.org/10.17268/sci.agropecu.2015.01.02
Volume Number	6
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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