Micro patterning of active proteins with perforated PDMS sheets (PDMS sieve).

Content Provider	Semantic Scholar
Author	Atsuta, Kyoko Noji, Hiroyuki Takeuchi, Shoji
Copyright Year	2004
Abstract	We propose a novel technique for patterning active proteins on a glass substrate using a perforated polydimethylsiloxane (PDMS) sheet-sieve. The sieve, which has tapering holes, is fabricated by spin-coating PDMS on a pyramidal-shaped mold. By means of this sieve, FITC (fluorescent isothiocyanate, bovine)-albumin was successfully spotted in a 5 x 5 microm(2) area in an array. The patterned spots were perfectly isolated, which eliminates the problem of non-specific binding of proteins to undesired areas. To show that proteins maintained their activity after the patterning, we used F(1)-ATPase biomolecular motors; their activity can easily be verified by observing their rotary motion after patterning. Selective patterning with three kinds of fluorescent micro beads indicated the possibility of patterning of different proteins on the same substrate by using the sieve.
File Format	PDF HTM / HTML
DOI	10.1039/B400774C
Alternate Webpage(s)	http://www.sanken.osaka-u.ac.jp/labs/smbio/sanken/ronbun_pdf/16AtsutaLabonChip04.pdf
PubMed reference number	15269800
Alternate Webpage(s)	https://doi.org/10.1039/B400774C
Volume Number	4
Issue Number	4
Journal	Lab on a chip
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article