NDLI: Enantiomeric Resolution and Determination of Clenbuterol by Hplc Technique in Plasma and Pharmaceutical Formulations Using Polysaccharide Chiral Stationary Phase and Uv Detection

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Enantiomeric Resolution and Determination of Clenbuterol by Hplc Technique in Plasma and Pharmaceutical Formulations Using Polysaccharide Chiral Stationary Phase and Uv Detection

Content Provider	Semantic Scholar
Author	Hefnawy, Mohamed M. Alshehri, Mona M. Sultan, Maha Taher Mostafa, Gamal A. E.
Copyright Year	2013
Abstract	Clenbuterol enantiomers were directly separated and determined in plasma and pharmaceutical formulation by a selective HPLC method using cellulose-based polysaccharide chiral stationary phase (CSP) known as OJ-RH. Enantiomeric resolution was achieved with a mobile phase consists of acetonitrile: 0.3M sodium perchlorate (16 %: 84 %), (v/v), a flow rate of 0.9 ml/min and a UV detection set at 247 nm. The method validated for its linearity, accuracy, and precision and robustness. The standard calibration curves were linear over the range of 0.5-50 μg/ml for each enantiomer with detection limit of 0.1 μg/ml. There was no significant difference between interand intra-day studies for each enantiomer which confirmed the reproducibility of the assay method. The method is highly specific where the co-formulated compounds did not interfere. The stability of clenbuterol enantiomers under high temperature was studied. The results showed that the drug is stable for at least 7 days at 80C. The mean extraction efficiency for R-(-)and S(+)clenbuterol from plasma was in the ranges 93-102 % at 7.5 40 μg/ml level for each enantiomers. The overall recoveries of clenbuterol enantiomers from pharmaceutical formulations were in the ranges 97 103 % with %RSD ranged from 1.81-2.35 %. The assay method proved to be chiral quality control for clenbuterol formulations by HPLC and to therapeutic drug monitoring.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.chalcogen.ro/1781_Hevnawy.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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