NDLI: Immunization of mice with fucosyl-GM1 conjugated with keyhole limpet hemocyanin results in antibodies against human small-cell lung cancer cells

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Immunization of mice with fucosyl-GM1 conjugated with keyhole limpet hemocyanin results in antibodies against human small-cell lung cancer cells

Content Provider	Semantic Scholar
Author	Cappello, Sarah Liu, Nancy X. Musselli, Cristina Brezicka, F. T. Livingston, Philip O. Ragupathi, Govindaswami
Copyright Year	1999
Abstract	Abstract Fucosyl-GM1 (Fuc-GM1) [Fucα1 → 2Galβ1 → 3GalNAcβ1 → 4(NeuAcα2-3)Galβ1 → 4Glcβ1 → O-Cer] is a small-cell-lung-cancer (SCLC)-associated ganglioside initially defined by the murine monoclonal antibody F12. On the basis of its known distribution, Fuc-GM1 is a potential target for active immunotherapy in SCLC patients. Fuc-GM1 has been extracted and purified from bovine thyroid. The immunogenicity of Fuc-GM1 was tested in mice either alone, mixed with carrier protein keyhole limpet hemocyanin (KLH) or covalently linked with KLH, plus immunological adjuvant QS-21. The Fuc-GM1-KLH conjugate plus QS-21 adjuvant was found to be optimal. It induced consistent IgM and IgG enzyme-linked immunosorbent assay (ELISA) titers against Fuc-GM1. These antibodies were strongly reactive with the strongly Fuc-GM1-positive rat hepatoma cell line H4-II-E, and they were moderately reactive with the moderately positive human SCLC cell line H146 by flow cytometry and complement-mediated lysis. Both ELISA and fluorescence-activated cell sorting (FACS) reactions were inhibited with Fuc-GM1or H4-II-E but not with the structurally related ganglioside GM1 or Fuc-GM1-negative colon cancer cell line LS-C. On the basis of these results, a vaccine comprising Fuc-GM1-KLH plus QS-21 is being prepared for testing in patients with SCLC.
Starting Page	483
Ending Page	492
Page Count	10
File Format	PDF HTM / HTML
DOI	10.1007/s002620050596
PubMed reference number	10602885
Journal	Medline
Volume Number	48
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1007/s002620050596
Alternate Webpage(s)	https://doi.org/10.1007/s002620050596
Journal	Cancer Immunology, Immunotherapy
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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