The α-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors

Content Provider	Semantic Scholar
Author	Naider, Fred R. Becker, Jeffrey M.
Copyright Year	2004
Abstract	Abstract Mating in Saccharomyces cerevisiae is initiated by the secretion of diffusible peptide pheromones that are recognized by G protein-coupled receptors (GPCR). This review summarizes the use of the α-factor (WHWLQLKPGQPMY) – GPCR (Ste2p) interaction as a paradigm to understand the recognition between medium-sized peptide hormones and their cognate receptors. Studies over the past 15 years have indicated that the α-factor is bent around the center of the pheromone and that residues near the amine terminus play a central role in triggering signal transduction. The bend in the center appears not to be rigid and this flexibility is likely necessary for conformational changes that occur as the receptor switches from the inactive to active state. The results of synthetic, biological, biochemical, molecular biological, and biophysical analyses have led to a preliminary model for the structure of the peptide bound to its receptor. Antagonists for Ste2p have changes near the N-terminus of α-factor, and mutated forms of Ste2p were discovered that appear to favor binding of these antagonists relative to agonists. Many features of this yeast recognition system are relevant to and have counterparts in mammalian cells.
Starting Page	1441
Ending Page	1463
Page Count	23
File Format	PDF HTM / HTML
DOI	10.1016/j.peptides.2003.11.028
Volume Number	25
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0196978104002943
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0196978104002943?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/j.peptides.2003.11.028
Journal	Peptides
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article