Loading...
Please wait, while we are loading the content...
Estudo da diversidade genética de Ralstonia solanacearum raça 2 no Brasil
| Content Provider | Semantic Scholar |
|---|---|
| Author | Pinheiro, Cássia Renata |
| Copyright Year | 2010 |
| Abstract | Ralstonia solanacearum Smith is a devastating plant pathogen with an global distribution and wide host range. It is the causal agent of bacterial wilts in more than 50 botanical families, and is considered one of the most important bacterial diseases in the world due to damage severity, systemic nature of infection and pathosystem characteristics that hinder their control. In banana causes moko-da-banana that can reach all plant organs in different developmental stages. For the moment, there are no efficient control measures or resistant varieties available that have any level of resistance or tolerance to disease, the potential damage to banana plantations is enormous, reaching up to 100%. An early detection system has been subject of research that aims to prevent disease progression, maintaining low the percentage of incidence. The characterization of the structure and genetic diversity of the pathogen is crucial to studies of epidemiology and orientation programs for disease management. The goal of this research was to molecularly characterize isolates of R. solanacearum according to new taxonomy of phylotypes and sequevars and analyze the isolates from three families of repetitive sequences (rep-PCR) and random primers (RAPD). We used 33 isolates from the collection of Embrapa Coastal Tablelands (CPATC) from various hosts, mostly associated with the banana crop. The characterization and determination of phylotypes and sequevars were made by Multiplex PCR and found that the collection is composed of CPATC isolates belonging to phylotypes II (88%) and phylotypes III (12%). The determination of sequevars by multiplex PCR was not satisfactory due to technical limitation of identifying only three multilocus genotypes. Significant relationships were isolated from the analysis of repetitive sequences, and with primer ERIC visualized clearly the separation of isolates of R. solanacearum according to race and the initiator REP possible to discern between phylotypes, which were the two most informative analyses. The technique of random primers (RAPD) was efficient to group the isolates according to their geographical origin, however requires a large number of molecular markers. With these results it was possible to characterize the pathogen and knowledge of the genetic structure of population as well as having direct application to the management of the disease contributes to understanding the evolution of this organism and its possible interactions between different isolates and their hosts. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repositorio.ufla.br/jspui/bitstream/1/1832/1/DISSERTA%C3%87%C3%83O_Estudo%20da%20diversidade%20gen%C3%A9tica%20de%20Ralstonia%20solanacearum%20ra%C3%A7a%202%20no%20Brasi.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
