Effects of pentachlorophenol and tetrachlorohydroquinone on mitogen-activated protein kinase pathways in Jurkat T cells.

Content Provider	Semantic Scholar
Author	Wispriyono, Bambang Matsuoka, Masato Igisu, Hideki
Copyright Year	2002
Abstract	When Jurkat human T cells were incubated with 20 microM of pentachlorophenol (PCP) or its metabolite, tetrachlorohydroquinone (TCHQ), for 10 hr, flow cytometric analyses revealed marked increase in the number of apoptotic cells. DNA fragmentation was also observed in these cells. TCHQ was more potent than PCP in causing apoptosis. After incubation with 20 microM TCHQ for 1 hr, all mitogen-activated protein kinases (MAPKs) examined [i.e., extracellular signal-regulated protein kinase (ERK), p38, and c-Jun NH(2)-terminal kinase (JNK)] were phosphorylated, whereas no clear phosphorylation was induced by PCP. TCHQ-induced apoptosis was markedly suppressed by treatment with a p38 inhibitor (SB203580) and mildly (but significantly) suppressed by treatment with a MAPK/ERK kinase inhibitor (U0126). When cells were treated with both inhibitors at the same time, TCHQ-induced apoptosis disappeared almost completely. PCP-induced apoptosis was also suppressed by SB203580 and/or U0126. Nevertheless, treatment with LL-Z1640-2, which inhibits JNK phosphorylation, did not suppress the apoptosis caused by either TCHQ or PCP. Thus, p38 and ERK appear to be important signal transduction pathways leading to apoptosis in a human T-cell line exposed to a ubiquitous pollutant or its metabolite in the general and occupational environment.
Starting Page	139
Ending Page	143
Page Count	5
File Format	PDF HTM / HTML
PubMed reference number	11836141
Volume Number	110
Journal	Environmental health perspectives
Language	English
Access Restriction	Open
Subject Keyword	Apoptosis DNA Fragmentation LL-C 10037alpha Mitogen-Activated Protein Kinases Mitogens Pentachlorophenol SB 203580 Signal Transduction Pathways U 0126 incubated
Content Type	Text
Resource Type	Article