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In Vitro Micropropagation of Stevia rebaudiana Bertoni
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yesmin, Sabina |
| Copyright Year | 2019 |
| Abstract | Multiple shoots were obtained from both shoot tips and nodal segments cultured on MS fortified with different concentrations of BAP and Kn singly or in combination with low concentration of NAA. Maximum number of shoots (9.28 ± 0.61) were found on MS supplemented with 1.5 mg/l BAP and 05 mg/l NAA. In vitro regenerated shoots were separated and transferred onto half and full-strength MS supplemented with different concentration of IBA, IAA and NAA for root induction. Full strength MS containing 0.2 mg/l IBA was found to be best for root induction where 93.33% shoots were rooted. In vitro regenerated plants grew normally without showing any morphological variation and flowered after 40 days of transplantation. Introduction Stevia rebaudiana Bertoni, commonly known as sweet herb or honey leaf is a small branched, semi-bushy, perennial shrub belongs to Asteraceae. Stevia is originated from the highlands of Paraguay and sections of Argentina and Brazil (Robinson 1930). Dr. Moises Santiago Bertoni, a South American Natural Scientist revealed stevia in 1888 and described its taxonomy (Sakaguchi and Kan 1982). This species is adapted to moist area and needs continuously wet places (Antonie and Greenwood 2000). The plant height of stevia is about 50 65 cm, leaves are sessile, oppositely arranged lanceolate to oblanceolate leaves, serrated towards apex. Stevia is one of the genera of 154 members of the family of Asteraceae (Robinson 1930). Stevia has medicinal properties which are used against antibacterial, anticandidal, antifungal, antiviral, cardio tonic, diuretic, hypoglycemic, vasodilator (Jitendra et al. 2012). Leaves of stevia are the source of diterpene glycosides, stevioside and rebaudioside. Stevioside is regarded as a valuable natural sweetening agent because of its relatively good taste and chemical stability (Yamada et al. 1985). *E-mail: . DOI: https://doi.org/10.3329/ptcb.v29i2.44516 |
| Starting Page | 277 |
| Ending Page | 284 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.3329/ptcb.v29i2.44516 |
| Volume Number | 29 |
| Alternate Webpage(s) | https://www.banglajol.info/index.php/PTCB/article/download/44516/34065 |
| Alternate Webpage(s) | https://doi.org/10.3329/ptcb.v29i2.44516 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
