NDLI: Polymorphic Protective Dps–DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering

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Polymorphic Protective Dps–DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering

Content Provider	Semantic Scholar
Author	Kamyshinsky, Roman Chesnokov, Yury Dadinova, Liubov A. Mozhaev, Andrey A. Orlov, Ivan A. Petoukhov, Maxim Orekhov, Anton S. Shtykova, Eleonora V. Vasiliev, Alexander N.
Copyright Year	2019
Abstract	Rapid increase of intracellular synthesis of specific histone-like Dps protein that binds DNA to protect the genome against deleterious factors leads to in cellulo crystallization-one of the most curious processes in the area of life science at the moment. However, the actual structure of the Dps-DNA co-crystals remained uncertain in the details for more than two decades. Cryo-electron tomography and small-angle X-ray scattering revealed polymorphous modifications of the co-crystals depending on the buffer parameters. Two different types of the Dps-DNA co-crystals are formed in vitro: triclinic and cubic. Three-dimensional reconstruction revealed DNA and Dps molecules in cubic co-crystals, and the unit cell parameters of cubic lattice were determined consistently by both methods.
File Format	PDF HTM / HTML
PubMed reference number	31888079
Journal	Medline
Volume Number	10
Alternate Webpage(s)	https://res.mdpi.com/d_attachment/biomolecules/biomolecules-10-00039/article_deploy/biomolecules-10-00039.pdf
Alternate Webpage(s)	https://doi.org/10.3390/biom10010039
Journal	Biomolecules
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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