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Glucagon-like peptide-1 potentiates glucose-stimulated insulin secretion via the transient receptor potential melastatin 2 channel.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Pang, Bo Kim, Sungjoon Li, Daiqing Ma, Zejun Sun, Bei Zhang, Xiaona Wu, Zhongming Chen, Liming |
| Copyright Year | 2017 |
| Abstract | The transient receptor potential melastatin 2 (TRPM2) channel, a Ca2+ permeable channel activated by cAMP, is expressed on pancreatic β-cells and is responsible for the regulation of insulin secretion. It is known that glucose-stimulated insulin secretion (GSIS) can be potentiated by glucagon like peptide-1 (GLP-1), and that the changes in the extracellular glucose concentration alter the levels of intracellular adenosine ATP and cAMP. The present study hypothesized that TRPM2 mediates the modulatory effect of GLP-1 on insulin secretion. The results demonstrated that silencing of TRPM2 eliminated GLP-1-enhanced insulin secretion, indicating the involvement of TRPM2 in this process. In addition, the results of current recordings of TRPM2 and measurement of the resulting insulin secretion in β-cells in the presence of GLP-1 and various concentrations of glucose suggest that GLP-1 regulates GSIS via the TRPM2 channel. Furthermore, inhibiting the activity or expression of TRPM2 attenuated GLP-1-induced GSIS. By using specific activators or inhibitors, the present study demonstrated that the two primary downstream effectors of the GLP-1 receptor, exchange protein directly activated by cAMP and protein kinase A, differentially influence GSIS and GLP-1-potentiated GSIS. In conclusion, the present study revealed the role of TRPM2 in GLP-1-regulated insulin secretion. The results of the present study provide a novel avenue for the prevention and treatment of diabetes and its complications. |
| Starting Page | 5219 |
| Ending Page | 5227 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/etm/14/5/5219/download |
| PubMed reference number | 29201240v1 |
| Alternate Webpage(s) | https://doi.org/10.3892/etm.2017.5136 |
| DOI | 10.3892/etm.2017.5136 |
| Journal | Experimental and therapeutic medicine |
| Volume Number | 14 |
| Issue Number | 5 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 8-chloro-cyclic adenosine monophosphate Adenosine Triphosphate Diabetes Mellitus Glucagon Glucagon-Like Peptide Receptors Glucagon-Like Peptide-1 Receptor Glucose Inhibition Protein Kinases TRPM3 protein, human insulin secretion |
| Content Type | Text |
| Resource Type | Article |
