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Localization of intestinal sucrase-isomaltase complex on the microvillous membrane by electron microscopy using nonlabeled antibodies
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nishi, Yoshinori Takesue, Yoshiki |
| Copyright Year | 1978 |
| Abstract | Microvillous vesicles isolated from rabbit small intestine showed a trilaminar membrane with a rather smooth surface, which was apparently not affected by papain solubilizing sucrase-isomaltase complex or by trypsin unable to solubilize it. When microvilous vesicles or trysinized ones were incubated with immunoglobulin G against the sucrase-isomaltase complex or monovalent fragments therefrom, an apparently continuous electron-opaque layer approximately 180 A in width appeared around the external surface of vesicles. Such a layer was not formed on papainized vesicles. Microvillous vesicles and trypsinized ones negatively stained with phosphotungate showed a great number of particles protruding approximately 150 A from the membrane surface, but papainized vesicles did not. The particles existed close to one another and appeared to form a particulate layer 150 A in width on the surface. The antibodies, whether they were divalent or monovalent, increased the width of the layer to approximately 200 A and obscured the fine particulate structure of intact and trypsinized vesicles. Papainized vesicles retained their smooth surface upon interaction with antibodies. These results, together with those with the Triton-solubilized sucrase- isomaltase complex (Nishi and Takesue, 1978), J. Ultra-struct. Res., 62:1- 12), indicate not only that sucrase-isomaltase complexes are located close to one another on the membrane, but also that they or at least their protein portions protrude approximately 150 A from the surface of the trilaminar membrane. |
| Starting Page | 516 |
| Ending Page | 525 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 721898v1 |
| Volume Number | 79 |
| Journal | The Journal of cell biology |
| Alternate Webpage(s) | http://jcb.rupress.org/content/jcb/79/2/516.full.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Oligo-1,6-Glucosidase Papain Particulate (substance) SUCRASE Small Intestinal Wall Tissue Sucrase-isomaltase deficiency, congenital Tissue membrane Vesicle (morphologic abnormality) incubated sucrase-isomaltase complex location width |
| Content Type | Text |
| Resource Type | Article |
