NDLI: DNA methylation modulates Salmonella enterica serovar Typhimurium virulence in Caenorhabditis elegans.

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DNA methylation modulates Salmonella enterica serovar Typhimurium virulence in Caenorhabditis elegans.

Content Provider	Semantic Scholar
Author	Oza, Javin P. Yeh, Jimmy B. Reich, Norbert O.
Copyright Year	2005
Abstract	Salmonella enterica serovar Typhimurium was previously shown to be virulent in Caenorhabditis elegans. Here we demonstrate that DNA adenine methyltransferase (DAM) modulates Salmonella virulence in the nematode, as it does in mice. After 5 days of continual exposure to bacteria, twice as many worms died when exposed to the wild-type than the dam-mutant strain of Salmonella. Similar trends in virulence were observed when worms were exposed to Salmonella strains for 5 h and transferred to the avirulent Escherichia coli OP50. While a 10-fold attenuation was observed in the absence of DAM, the dam-strain was still able to infect and persist in the host worm. Our results further support the use of C. elegans as an accessible and readily studied animal model of bacterial pathogenesis. However, our results suggest that crucial host responses differ between the murine and nematode models. Additionally, we carried out preliminary liquid culture based experiments with the long term goal of developing high throughput animal based screens of DAM inhibitors.
Starting Page	1
Ending Page	4
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/femsle/245/1/10.1016/j.femsle.2005.02.030/2/245-1-53.pdf?Expires=1497943395&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=LRWlz-~AAKWJc4kNKRAdKyiXNSRGQyLGxqiK9C1YjuAAEuop78Uf2MMxtehtgoleUoLLu2hfGAs0F99F2wSaQ4HXatSxbM0PDNgUvadPtNM1DjiQZc17italBskeIQMTrNgT3CTUKM8BwJQWjK28A530u6ZlraPEDdBcTuq5h3sQU7kMNS79ca2lGEroXdtWUKBK~1WiQ5o0VQCL5Z~Z4HGdubSWKCaQpFTYOzqk55Iz7JgekAMu4Smfx5mS0YwIC5yhcYIL6qDobAXc9b0gzxaRjW9lhOAW8QU0at9HXSs07ygOFwVeDhrRNPEyYoprgnwKkBQwEsV5COj0zQCXxg__
PubMed reference number	15796979v1
Volume Number	245
Issue Number	1
Journal	FEMS microbiology letters
Language	English
Access Restriction	Open
Subject Keyword	Adenine Caenorhabditis elegans Cessation of life Methyltransferase Nematodes Salmonella enterica Serotype Virulence
Content Type	Text
Resource Type	Article

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