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Antioxidative and antimicrobial activities of different solvent extracts of Moringa oleifera : an in vitro evaluation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tekle, Esayas Welday Sahu, N. P. |
| Copyright Year | 2015 |
| Abstract | Aqueous, methanolic (80%) and ethanolic (70%) extracts of the leaves, flower, stem bark and pod/fruit (denoted as AL, AF, AB, AP, ML, MF, MB, MP, EL, EF, EB and EP for aqueous leaf, aqueous flower, aqueous bark, aqueous pod, methanolic leaf, methanolic flower, methanolic bark, methanolic pod, ethanolic leaf, ethanolic flower, ethanolic bark and ethanolic pod extracts, respectively) of Moringa oleifera were evaluated for their antioxidative activities and antimicrobial properties. 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity (%), total phenolic contents (TPC) and ferric reducing antioxidant power (FRAP) were used as a means of evaluating the in vitro anti-oxidative activities of the extracts. All the extracts showed a dose-dependent activity with each method. There was a significant positive correlation (P<0.01) between DPPH values and TPC (r 2 = 0.76) as well as between TPC and FRAP value (r 2 = 0.93). With the DPPH and FRAP methods, EL and AB showed highest and lowest (P<0.05) overall activity, respectively. Moreover, EL exhibited highest activity with all the three methods starting from the lowest concentrations. At the higher two concentrations (7.5 and 10 mg/ml), EF showed highest activity (P<0.05) as compared to all the other extracts and a standard antioxidant, BHT. Anti-microbial activity of the extracts using the disc diffusion method and minimum inhibitory concentration (MIC) assay revealed that ethanolic extracts from all parts of moringa showed highest activity against Aeromonas hydrophila and Micrococcus luteus on concentration dependent manner than the aqueous extract counterpart. Although all the extracts exhibited considerable anti-oxidant activities with either one or both of the assays, EL and EF showed the best results and can be recommended as natural anti-oxidants in food/feed preparation and formulation. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ijsrp.org/research-paper-0515/ijsrp-p4131.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |