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Evidence for lipopolysaccharide-induced differentiation of RAW 264 ⋅ 7 murine macrophage cell line into dendritic like cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ajiv S. Yang K. Aniel Ewis |
| Copyright Year | 2003 |
| Abstract | Effect of lipopolysaccharide (LPS) on RAW264⋅7 macrophage cell line was studied. LPS-treated RAW264⋅7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 μg/ml), almost 70% RAW264⋅7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell activation (B7⋅1, B7⋅2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW264⋅7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repository.ias.ac.in/51792/1/98-PUB.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
