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Polycomb group suppressor of zeste 12 links heterochromatin protein 1alpha and enhancer of zeste 2.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yamamoto, Ken Sonoda, Miki Inokuchi, Junichi Shirasawa, Senji Sasazuki, Takehiko |
| Copyright Year | 2004 |
| Abstract | Drosophila suppressor of zeste 12 (Su(z)12) is a Polycomb group (PcG) transcriptional repressor and is present in E(z)-ESC, a multiprotein complex with methylation activity specific for lysine 9 and 27 of histone H3. Although PcG- and heterochromatin-mediated gene silencing have been considered distinct, mutant flies of Su(z)12 showed not only homeotic transformation but also position effect variegation. We now report that the mammalian SU(Z)12 directly interacts with heterochromatin protein 1alpha (HP1alpha) and PcG enhancer of zeste 2 (EZH2), the mammalian counterpart of E(z), in vitro and in vivo. Two distinct domains in SU(Z)12 are involved in these interactions, the region between the zinc finger motif and the VEFS (VRN2-EMF2-FIS2-Su(z)12) box for HP1alpha (amino acid residues 479-536) and the VEFS box for EZH2 (amino acid residues 600-639), which are not mutually exclusive. Interestingly this region of the VEFS box has been shown to be critical for the phenotype of the Su(z)12 mutant fly. In addition SU(Z)12 represses transcription activity in the presence of HP1alpha in a reporter assay. These results provide a molecular explanation for the functional link of these epigenetic silencing processes mediated by Su(z)12. |
| Starting Page | 309 |
| Ending Page | 319 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jbc.org/content/279/1/401.full.pdf |
| PubMed reference number | 14570930v1 |
| Volume Number | 279 |
| Issue Number | 1 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Diptera EZH2 gene Enhancer of transcription Epigenetic Silencing Gene Silencing Heterochromatin Histone H3 Histones Lysine Mammals Methylation Polycomb-Group Proteins Position Effect Variegation Protein Domain Transcription Repressor/Corepressor Transcription, Genetic Zinc Fingers |
| Content Type | Text |
| Resource Type | Article |
