Development of multiplex PCR method for simultaneous detection of four events of genetically modified maize: DAS-59122-7, MIR604, MON863 and MON88017.

Content Provider	Semantic Scholar
Author	Oguchi, Taichi Onishi, Mari Mano, Junichi Akiyama, Hiroshi Teshima, R. Futo, Satoshi Furui, Satoshi Kitta, Kazumi
Copyright Year	2010
Abstract	A novel multiplex PCR method was developed for simultaneous event-specific detection of four events of GM maize, i.e., DAS-59122-7, MIR604, MON88017, and MON863. The single laboratory examination of analytical performance using simulated DNA mixtures containing GM DNA at various concentrations in non-GM DNA suggested that the limits of detection (LOD) of the multiplex PCR method were 0.16% for MON863, MIR604, and MON88017, and 0.078% for DAS-59122-7. We previously developed a nonaplex (9plex) PCR method for eight events of GM maize, i.e., Bt11, Bt176, GA21, MON810, MON863, NK603, T25, and TC1507. Together with the nonaplex PCR method, the newly developed method enabled the detection and identification of eleven GM maize events that are frequently included in commercial GM seed used in Japan. In addition, this combinational analysis may be useful for the identification of combined event products of GM maize.
Starting Page	92
Ending Page	100
Page Count	9
File Format	PDF HTM / HTML
DOI	10.3358/shokueishi.51.92
PubMed reference number	20595789
Journal	Medline
Volume Number	51
Issue Number	3
Journal	Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/shokueishi/51/3/51_3_92/_pdf
Alternate Webpage(s)	https://doi.org/10.3358/shokueishi.51.92
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article