NDLI: Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs).

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Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs).

Content Provider	Semantic Scholar
Author	Barnes, Richard H. Chun, Tae-Hwa
Copyright Year	2016
Abstract	The isolation of adipose-derived stem cells (ASCs) is an important method in the field of adipose tissue biology, adipogenesis, and extracellular matrix (ECM) remodeling. In vivo, ECM-rich environment consisting of fibrillar collagens provides a structural support to adipose tissues during the progression and regression of obesity. Physiological ECM remodeling mediated by matrix metalloproteinases (MMPs) plays a major role in regulating adipose tissue size and function(1,2). The loss of physiological collagenolytic ECM remodeling may lead to excessive collagen accumulation (tissue fibrosis), macrophage infiltration, and ultimately, a loss of metabolic homeostasis including insulin resistance(3,4). When a phenotypic change of the adipose tissue is observed in gene-targeted mouse models, isolating primary ASCs from fat depots for in vitro studies is an effective approach to define the role of the specific gene in regulating the function of ASCs. In the following, we define an immunomagnetic separation of Sca1(high) ASCs.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.jove.com/pdf-materials/53890/jove-materials-53890-immunomagnetic-separation-fat-depot-specific-sca1high-adipose-derived
PubMed reference number	27583550v1
Alternate Webpage(s)	https://doi.org/10.3791/53890
DOI	10.3791/53890
Journal	Journal of visualized experiments : JoVE
Volume Number	114
Language	English
Access Restriction	Open
Subject Keyword	Adipogenesis Adipose tissue Body tissue Collagen Extended Release Depot Dosage Form Extracellular Matrix Fibrosis ISOLATE COMPOUND Immunomagnetic Separation Matrix Metalloproteinases Metabolic Process, Cellular Metalloproteases Non-Fibrillar Collagens Platelet Glycoprotein 4, human Stem cells
Content Type	Text
Resource Type	Article

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