NDLI: Multiplex SNP-SCALE: a cost-effective medium-throughput single nucleotide polymorphism genotyping method.

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Multiplex SNP-SCALE: a cost-effective medium-throughput single nucleotide polymorphism genotyping method.

Content Provider	Semantic Scholar
Author	Kenta, Tanaka Gratten, Jake Haigh, Nathan S. Hinten, Gavin N. Slate, Jon Butlin, Roger K. Burke, Terry A.
Copyright Year	2008
Abstract	We describe a convenient, cost-effective and flexible medium-throughput single nucleotide polymorphism (SNP) genotyping method, Multiplex SNP-SCALE, which enables the simultaneous amplification by polymerase chain reaction (PCR) of up to 25 (or potentially more) loci followed by electrophoresis in an automated DNA sequencer. We extended the original SNP-SCALE method to include (i) use of a commercial multiplex PCR kit, (ii) a four-dye system, (iii) much-reduced (2-µL) reaction volumes, (iv) drying down of template DNA before PCR, (v) use of pig-tailed primers, (vi) a PCR product weighting system, (vii) a standard optimized touchdown PCR thermocycling programme, and (viii) software (SNP-SCALE Primer Designer) that automatically designs suitable SNP-SCALE primers for a batch of loci. This new protocol was validated for different types of SNPs. The method is cost- and time-effective for medium-scale evolutionary and ecological projects involving 10s to 100s of loci.
File Format	PDF HTM / HTML
DOI	10.1111/j.1755-0998.2008.02190.x
PubMed reference number	21586010
Journal	Medline
Volume Number	8
Issue Number	6
Alternate Webpage(s)	http://molecolab.group.shef.ac.uk/wp-content/uploads/kenta_proof_7may08.pdf
Alternate Webpage(s)	https://doi.org/10.1111/j.1755-0998.2008.02190.x
Journal	Molecular ecology resources
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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