NDLI: The Royal Society of Self-assembly of suspended collagen fi lms and their viability as cell culture substrates †

Content Provider	Semantic Scholar
Author	Roberts, Megan Johnson Bhatt, Niharika Joshi Voge, Chris M. Meshot, Eric R. Stegemannc, Jan P. Hart, A. John
Copyright Year	2013
Abstract	We present the fabrication and mechanical properties of thin collagen networks self-assembled in a suspended configuration over micropost arrays. These collagen “canopies” were formed on arrays of microposts made of PDMS, silicon, and vertically aligned carbon nanotubes (CNT). We reversibly loaded the canopy to an in-plane stress of 32 MPa. We found that human dermal fibroblasts (HDFb) proliferate on the canopy substrates for up to 7 days. This versatile fabrication method for suspended extracellular matrix (ECM) films may enable the development of new assays to probe cell–ECM interactions, along with integration of microelectronic probes.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://mechanosynthesis.mit.edu/journals/086_roberts_13_collagen.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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