Disseny de marcadors moleculars per a l'estudi de l'herència materna en albergínia (Solanum melongena) i espècies silvestres relacionades.

Content Provider	Semantic Scholar
Author	Cortés, Trigo Marina, Alba
Copyright Year	2019
Abstract	The objective of this work is the study of the maternal inheritance of the chloroplast in Solanum melongena and other related wild species, more or less close, with which it is crossed for its improvement. It has been discovered that maternal inheritance can mainly affect male sterility, as well as other phenotypic characteristics, so it is interesting to develop markers to determine which parent contributes with this type of material. The aim is to design molecular markers from DNA sequences that are not coding for the chloroplast, belonging to different regions. To do this, the DNA of the different species to be studied will be collected and will be amplified and sequenced, to obtain the sequences for its study. With this information, the consensus sequences necessary for the development of markers will be obtained through the use of different computer programs. Subsequently, CAPS will be designed through the search for restriction enzymes that allow to differentiate maternal inheritance between wild species and Solanum melongena. On the other hand, nucleotide changes that do not result in different restriction cleavage sites will be checked by the HRM technique. These markers will be tested in F1 individuals where Solanum melongena or wild as a mother has been used, as well as in breeding populations where a wild parent has been used.
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Alternate Webpage(s)	https://riunet.upv.es/bitstream/handle/10251/126820/Trigo%20-%20Dise%C3%B1o%20de%20marcadores%20moleculares%20para%20el%20estudio%20de%20la%20herencia%20materna%20en%20berenjena%20(Sol....pdf?isAllowed=y&sequence=8
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article