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Strychnine-insensitive binding of [3H]glycine to synaptic membranes in rat brain, treated with triton X-100
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ogita, Kiyokazu Suzuki, Takayoshi Yoneda, Yukio |
| Copyright Year | 1989 |
| Abstract | Binding of radiolabelled glycine, a putative inhibitory neurotransmitter in mammalian lower central structures, was examined by using the synaptic membranes of the brain of rat, treated with Triton X-100. This treatment with Triton markedly potentiated the binding of [3H]glycine detected at 2 degrees C and 30 degrees C. However, this binding was not affected by three different convulsants, strychnine, picrotoxin and bicuculline. The binding was saturable at 2 degrees C, with increasing concentrations of [3H]glycine up to 1 microM. Scatchard analysis revealed that the binding sites consisted of a single component with a Kd of 202 nM and a Bmax of 1.74 pmol/mg protein. The binding was inhibited, not only by various amino acids structurally related to glycine, including D- and L-serine and D-, L- and beta-alanine, but was also eliminated by some peptides containing glycine, such as gamma-D- and gamma-L-glutamylglycine, glycine methylester and N-methyl-glycine. In addition, the strychnine-insensitive binding of [3H]glycine was significantly abolished by numerous quinoxaline antagonists for excitatory amino acid receptors in the brain. These results suggest that synaptic membranes of brain, treated with Triton X-100, are useful to detect the strychnine-insensitive binding of [3H]glycine and superior to untreated membranes in terms of the freedom from the confounding effects of some endogenous amino acids. |
| Starting Page | 1263 |
| Ending Page | 1270 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0028-3908(89)90220-7 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0028390889902207 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0028390889902207?dgcid=api_sd_search-api-endpoint |
| PubMed reference number | 2687715 |
| Alternate Webpage(s) | https://doi.org/10.1016/0028-3908%2889%2990220-7 |
| Journal | Medline |
| Volume Number | 28 |
| Journal | Neuropharmacology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
