Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design

Content Provider	Semantic Scholar
Author	Jamil, Zadariana Annuar, Mohamad Suffian Mohamad Ibrahim, Shaliza Vikineswary, Sabaratnam
Copyright Year	2008
Abstract	Abstract Phototrophic hydrogen production by indigenous purple non-sulfur bacteria, Rhodopseudomonas palustris PBUM001 from palm oil mill effluent (POME) was optimized using response surface methodology (RSM). The process parameters studied include inoculum sizes (% v/v), POME concentration (% v/v), light intensity (klux), agitation (rpm) and pH. The experimental data on cumulative hydrogen production and COD reduction were fitted into a quadratic polynomial model using response surface regression analysis. The path to optimal process conditions was determined by analyzing response surface three-dimensional surface plot and contour plot. Statistical analysis on experimental data collected following Box-Behnken design showed that 100% (v/v) POME concentration, 10% (v/v) inoculum size, light intensity at 4.0 klux, agitation rate at 250 rpm and pH of 6 were the best conditions. The maximum predicted cumulative hydrogen production and COD reduction obtained under these conditions was 1.05 ml H2/ml POME and 31.71% respectively. Subsequent verification experiments at optimal process values gave the maximum yield of cumulative hydrogen at 0.66 ± 0.07 ml H2/ml POME and COD reduction at 30.54 ± 9.85%.
Starting Page	7502
Ending Page	7512
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1016/j.ijhydene.2009.05.116
Volume Number	34
Alternate Webpage(s)	https://umexpert.um.edu.my/file/publication/00002687_44609.pdf
Alternate Webpage(s)	http://eprints.um.edu.my/9433/1/Optimization_of_phototrophic_hydrogen_production_by_Rhodopseudomonas_palustris_PBUM001_via_statistical_experimental_design.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.ijhydene.2009.05.116
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article