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Development of a diffraction imaging flow cytometer.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jacobs, Kenneth M. Lu, Jun Q. Hu, Xin-Hua |
| Copyright Year | 2009 |
| Abstract | Diffraction images record angle-resolved distribution of scattered light from a particle excited by coherent light and can correlate highly with the 3D morphology of a particle. We present a jet-in-fluid design of flow chamber for acquisition of clear diffraction images in a laminar flow. Diffraction images of polystyrene spheres of different diameters were acquired and found to correlate highly with the calculated ones based on the Mie theory. Fast Fourier transform analysis indicated that the measured images can be used to extract sphere diameter values. These results demonstrate the significant potentials of high-throughput diffraction imaging flow cytometry for extracting 3D morphological features of cells. |
| Starting Page | 125 |
| Ending Page | 125 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://bmlaser.physics.ecu.edu/literature/2009%2010_flow%20cytometer%20with%20sphere%20imaging%20letter.pdf |
| PubMed reference number | 19794790v1 |
| Alternate Webpage(s) | https://doi.org/10.1364/OL.34.002985 |
| DOI | 10.1364/ol.34.002985 |
| Journal | Optics letters |
| Volume Number | 34 |
| Issue Number | 19 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Diameter (qualifier value) Flow Cytometry Polystyrenes automated flow cytometers |
| Content Type | Text |
| Resource Type | Article |