NDLI: Microfluidic nanoplasmonic-enabled device for multiplex DNA detection.

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Microfluidic nanoplasmonic-enabled device for multiplex DNA detection.

Content Provider	Semantic Scholar
Author	Peng, H. Strohsahl, Christopher M. Miller, Benjamin LeRoy
Copyright Year	2012
Abstract	We describe a rapid, quantitative, multiplex, self-labelled, and real-time DNA biosensor employing Ag nanoparticle-bound DNA hairpin probes immobilized in a microfluidic channel. Capture of complementary target DNAs by the microarrayed DNA hairpin probes results in a positive fluorescence signal via a conformational change of the probe molecules, signalling the presence of target DNAs. The device's capability for quantitative analyses was evaluated and a detection time as low as 6 min (with a target flow rate of 0.5 μl min(-1)) was sufficient to generate significant detection signals. This detection time translates to merely 3 μl of target solution consumption. An unoptimized sensitivity of 500 pM was demonstrated for this device.
Starting Page	451
Ending Page	452
Page Count	2
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.rsc.org/suppdata/lc/c2/c2lc21114a/c2lc21114a.pdf
PubMed reference number	22301735v1
Alternate Webpage(s)	https://doi.org/10.1039/c2lc21114a
DOI	10.1039/c2lc21114a
Journal	Lab on a chip
Volume Number	12
Issue Number	6
Language	English
Access Restriction	Open
Subject Keyword	Artificial nanoparticles Fluorescence Microfluidics Silver biological signaling
Content Type	Text
Resource Type	Article

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