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Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liu, Zhenhua Shen, Li Sun, Xiaoya Zhou, Jinwei Wu, Shiliang |
| Copyright Year | 2011 |
| Abstract | β-1,3-N-acetylglucosaminyltransferase-8 (β3Gn-T8) is the most recently identified enzyme in the β3Gn-T family, but its biological function is poorly understood. To elucidate the effects of β3Gn-T8 on gastric cancer behavior, β3Gn-T8 was down-regulated in AGS cells using small interfering RNA (siRNA). The mRNA and protein expression levels of β3Gn-T8 were detected using RT-PCR and Western blotting, respectively, and sequence-specific inhibition using siRNA was also measured using RT-PCR in human SPCA-1 and SGC-7901 cells. The cell proliferation rate was determined using MTT and the percentage of apoptotic cells was measured using flow cytometry. AGS cells transfected with β3Gn-T8 siRNA were subcutaneously transplanted into nude mice and tumorigenicity was assessed. The siRNA efficiently suppressed β3Gn-T8 expression in AGS cells, and the down-regulation of β3Gn-T8 caused significant inhibition of tumor cell growth in vitro. The apoptotic rate of AGS cells increased to 10.13% 48 h after siRNA transfection, which was five times that of the control cells. Furthermore, the knockdown of β3Gn-T8 expression reduced the tumorigenicity of gastric cancer cells in nude mice, suggesting that β3Gn-T8 has potential as a gastric cancer therapeutic target. |
| Starting Page | 497 |
| Ending Page | 503 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.3892/mmr.2011.448 |
| PubMed reference number | 21468598 |
| Journal | Medline |
| Volume Number | 4 |
| Issue Number | 3 |
| Alternate Webpage(s) | https://www.spandidos-publications.com/mmr/4/3/497/download |
| Alternate Webpage(s) | https://doi.org/10.3892/mmr.2011.448 |
| Journal | Molecular medicine reports |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
