NDLI: Stability-indicating High-performance Liquid Chromatography Method for Simultaneous Determination of Aminophylline and Chlorpheniramine Maleate in Pharmaceutical Formulations

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Stability-indicating High-performance Liquid Chromatography Method for Simultaneous Determination of Aminophylline and Chlorpheniramine Maleate in Pharmaceutical Formulations

Content Provider	Semantic Scholar
Author	Ahmed, Mahmood Maḥmūd, T̤āriq Qadir, Muhammad Abdul Nadeem, Kashif Saleem, Amina
Copyright Year	2015
Abstract	The present work deals with the development and validation of method for simultaneous determination of antihistaminic drugs in pharmaceutical formulations. A precise, specific and accurate reverse phase-high-performance liquid chromatography method for the simultaneous measurement of aminophylline and chlorpheniramine maleate was developed. The separation of drugs was achieved on C-18 (5 μm, 250×4.6 mm) high-performance liquid chromatography column. The runtime for analysis was 10 min. Mobile phase is mixture containing dilute H2SO4:methanol (60:40% v/v) with flow rate adjusted at 1.5 ml/min. The detection of components was performed at a wavelength of 264 nm. Retention times of aminophylline and chlorphinramine maleate were found to be 2.00 and 3.25 min, respectively. Linearity was found in the range of 16-24 μg/ml for chlorpheniramine maleate and 102.4-153.6 μg/ml for aminophylline with a correlation coefficient of 0.9998 and 0.9996, respectively. High peak purity index of 99.99% indicated the complete separation of analytes in the presence of degradation products is justification of method stability. Linearity, accuracy, specificity, precision and robustness studies were performed for method validation.
Starting Page	515
Ending Page	521
Page Count	7
File Format	PDF HTM / HTML
PubMed reference number	26798164
Journal	Medline
Volume Number	77
Alternate Webpage(s)	http://www.ijpsonline.com/articles/stabilityindicating-highperformance-liquid-chromatography-method-for-simultaneous-determination-of-aminophylline-and-chl.pdf
Alternate Webpage(s)	https://doi.org/10.4103/0250-474X.169042
Journal	Indian journal of pharmaceutical sciences
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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