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Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ponniah, Jeyaletchumi Robin, T. Delvin P. Margaret S. Radu, Son Ghazali, Farinazleen Mohamad Cheah, Yoke Kqueen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep Kumar |
| Copyright Year | 2010 |
| Abstract | The aim of this study was to assess the most probable number-polymerase chain reaction (MPN-PCR) technique for detection of Listeria monocytogenes in salad vegetables in comparison with reference EN ISO 11290-2 and Food Drug Administration Bacteriological Analytical Manual method using artifcial and naturally contaminated samples. Based on recovery of L. monocytogenes from artifcially contaminated samples, MPN-PCR showed a moderate correlation (R=0.67) between spiking concentration and microbial levels which was better than the FDA-BAM method (R=0.642) and ISO 11290-2:1998 method (R=0.655). With naturally contaminated samples, it was found that L. monocytogenes was detected in 25% of the vegetable samples using MPN-PCR; 15% of the samples by the FDA-BAM method and 8% of samples using ISO 11290-2:1998 method. Overall, MPN-PCR was found to be a rapid and reliable method that could facilitate the enumeration of L. monocytogenes in vegetables. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://psasir.upm.edu.my/16411/1/16411.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
