“On-line” measurement of acetylcholinesterase release from the substantia nigra of the freely-moving guinea-pig

Content Provider	Semantic Scholar
Author	Taylor, S. J. Jones, Samantha A. Haggblad, J. Greenfield, Susan Adele
Copyright Year	1990
Abstract	Acetylcholinesterase is released from dopaminergic cells within the substantia nigra. The functional significance of this phenomenon has been studied in the freely-moving animal by a novel system for measuring acetylcholinesterase release from the substantia nigra "on-line" and in vivo. In the unanaesthetized guinea-pig the amount of acetylcholinesterase released was significantly greater than during anaesthesia, and release occurred in a more pulsatile manner. In addition, release of acetylcholinesterase could be evoked by either pharmacological or physiological manipulations, i.e. (1) a depolarizing concentration of potassium ions administered locally; (2) metamphetamine, administered systematically, which also resulted in increased locomotor activity; (3) drinking behaviour, elicited by presentation of a water bottle. Although all three treatments were accompanied by an increase in acetylcholinesterase release within the substantia nigra, potassium-evoked release did not cause any detectable change in behaviour. It is therefore suggested that release of the protein acetylcholinesterase within the substantia nigra is not necessarily a direct cause of locomotor activity: rather, it reflects diverse sensorimotor events.
Starting Page	71
Ending Page	76
Page Count	6
File Format	PDF HTM / HTML
DOI	10.1016/0306-4522(90)90193-8
PubMed reference number	2243598
Journal	Medline
Volume Number	37
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/0306452290901938
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/0306452290901938?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/0306-4522%2890%2990193-8
Journal	Neuroscience
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article