Molecular and Physiological Studies on Basil (Ocimum basilicum L.) Under Cadmium Stress

Content Provider	Semantic Scholar
Author	Khavari-Nejad, R. A. Najafi, Farzaneh Angaji, S. Abdolhamid Shafiei, Safiyeh
Copyright Year	2013
Abstract	Cadmium toxicity is a serious problem in agriculture. Cadmium accumulation in soils may come from different sources, including air pollutants and soil applications of commercial fertilizers, sewage sludge, manure and lime. The cadmium exposure causes oxidative stress. Phenol and flavonoid are involved in this stress. The effect of different concentrations of cadmium chloride (0, 25, 50 and 75 µM) on the phenol, flavonoid and proline contents in Ardestan and Isfahan cultivars of basil (Ocimum basilicum L.) were studied. The samples were harvested after 18 days of treatment.With increasing concentration of cadmium chloride, the content of phenol, flavonoid and proline increased in both of the cultivars. The increase in Ardestan cultivar was more pronounced than Isfahan cultivar. According to the results, it appears that Ardestan and Isfahan cultivars are both tolerant to cadmium stress but Ardestan cultivar may do more compare to other one. Effective methods are needed to identify those genes that are differentially expressed under cadmium condition. The differential display PCR (DD-PCR) was conceived to allow the identification and molecular cloning of differentially expressed genes. This technique was devised to amplify messenger RNAs and display their 3' termini on polyacrylamide gels. It was concluded that the plant responded to the stress conditions by altering the gene expression of regulatory metabolic processes, especially the photosynthesis.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://idosi.org/aejaes/jaes13(6)13/3.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article