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Covalent functionalization of graphene oxide with d-mannose: evaluating the hemolytic effect and protein corona formation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sousa, Marcelo De Martins, Carlos Henrique Zanini Franqui, Lidiane Silva Fonseca, Leandro Carneiro Delite, Fabrício S. Lanzoni, Evandro Martin Martinez, Diego Stéfani Teodoro Alves, Oswaldo Luiz |
| Copyright Year | 2018 |
| Abstract | In this work, graphene oxide (GO) was covalently functionalized with d-mannose (man-GO) using mannosylated ethylenediamine. XPS (C1s and N1s) confirmed the functionalization of GO through the binding energies at 288.2 eV and 399.8 eV, respectively, which are attributed to the amide bond. ATR-FTIR spectroscopy showed an increase in the amine bond intensity, at 1625 cm-1 (stretching C[double bond, length as m-dash]O), after the functionalization step. Furthermore, the man-GO toxicity to human red blood cells (hemolysis) and its nanobiointeractions with human plasma proteins (hard corona formation) were evaluated. The mannosylation of GO drastically reduced its toxicity to red blood cells. SDS-PAGE analysis showed that the mannosylation process of GO also drastically reduced the amount of the proteins in the hard corona. Additionally, proteomics analysis by LC-MS/MS revealed 109 proteins in the composition of the man-GO hard corona. Finally, this work contributes to future biomedical applications of graphene-based materials functionalized with active biomolecules. |
| Starting Page | 2803 |
| Ending Page | 2812 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/C7TB02997G |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/c7/tb/c7tb02997g/c7tb02997g1.pdf |
| PubMed reference number | 32254233 |
| Alternate Webpage(s) | https://doi.org/10.1039/C7TB02997G |
| Journal | Medline |
| Volume Number | 6 |
| Issue Number | 18 |
| Journal | Journal of materials chemistry. B |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
