NDLI: Low rhodamine 123 retention identifies long-term human hematopoietic stem cells within the Lin-CD34+CD38- population.

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Low rhodamine 123 retention identifies long-term human hematopoietic stem cells within the Lin-CD34+CD38- population.

Content Provider	Semantic Scholar
Author	McKenzie, Joby L. Takenaka, Katsuto Gan, Olga I. Doedens, Monica Dick, John E.
Copyright Year	2007
Abstract	Progress to uncover the molecular and cellular regulators that govern human hematopoietic stem cell (HSC) fate has been impeded by an inability to obtain highly purified fractions of HSCs. We report that the rhodamine 123 (Rho 123) dye effluxing fraction of the Lin-CD34+CD38- population contains SCID-repopulating cells (SRCs) capable of long-term repopulation in primary nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Purification based on Rho uptake led to a 4-fold enrichment of SRCs in the Lin-CD34+CD38- fraction, with a frequency of 1 SRC in 30 Lin-CD34+CD38-Rholo cells. The Lin-CD34+CD38-Rholo fraction also possesses long-term self-renewal capacity as measured by serial transplantation totaling more than 20 weeks. We conclude that Rho dye efflux provides an additional means of purifying human HSCs in the quest to achieve homogeneous populations of primitive cells for both experimental and therapeutic applications.
Starting Page	37
Ending Page	48
Page Count	12
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.bloodjournal.org/content/bloodjournal/109/2/543.full.pdf?sso-checked=true
PubMed reference number	16990597v1
Volume Number	109
Issue Number	2
Journal	Blood
Language	English
Access Restriction	Open
Subject Keyword	Diabetes Mellitus Dyes Hematopoietic stem cells Immunologic Deficiency Syndromes Lin-Buspirone Lobular Neoplasia Rhodamine 123 SCID Mice SCID-Repopulating Cell
Content Type	Text
Resource Type	Article

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