NDLI: Mutational and in vivo methylation analysis of F-factor PifC protein binding to the pif operator and the region containing the primary origin of mini-F replication.

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Mutational and in vivo methylation analysis of F-factor PifC protein binding to the pif operator and the region containing the primary origin of mini-F replication.

Content Provider	Semantic Scholar
Author	Miller, Jeff Malamy, Michael H.
Copyright Year	1986
Abstract	We have used in vivo methods to identify multiple DNA-binding sites for the negatively autoregulated mini-F replication factor PifC. Sequence analysis of pif operator constitutive mutants, isolated as insensitive to repression by PifC, establishes the structure of pifO. This site contains a 17-base-pair (bp) region of dyad symmetry with 7-bp perfect inverted repeats separated by 3 bp. In vivo DNA methylation studies with dimethyl sulfate show that the reactivity of five of six guanine residues in the pifO region is altered in the presence of PifC protein. In addition, there are several sites of PifC-dependent methylation enhancement and protection upstream of pifO within repeated sequences bearing homology to pifO. The significance of the repeated PifC binding sequences and their relationship to the primary origin of mini-F replication (oriV1) are discussed.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.83.5.1433
Alternate Webpage(s)	http://www.pnas.org/content/83/5/1433.full.pdf
PubMed reference number	3513175
Alternate Webpage(s)	https://doi.org/10.1073/pnas.83.5.1433
Journal	Medline
Volume Number	83
Issue Number	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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