NDLI: Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents

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Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents

Content Provider	Semantic Scholar
Author	Fatemeh, Sarvi Najafabadi, S. Adel Hashemi Faraahani, E. Vasheghani Alsadati, S. A. Shoja
Copyright Year	2008
Abstract	Methoxy poly(ethylene glycol) (mPEG) with molecular mass of 5 kDa activated with succinimidyl carbonate and cyanuric chloride, separately was covalently attached to human red blood cells (RBCs). Inhibition of agglutination by blood-type specific antisera (anti-D) was employed to evaluate the effect of the polymer coating. The remaining single cells after incubation with anti-D sera were counted using a simple hemocytometer (Improved Neubauer Ruling). The extent of surface coating was evaluated by addition of FITC labeled-anti-D to the cells and recording the fluorescence intensity ratio of FITC-anti-D bound cells of the PEG-RBCs versus control (uncoated) RBCs. The morphology of RBCs was evaluated by scanning electron microscopy (SEM). The effect of polymer coating, based on the immunological response of RBCs, using two kinds of activated mPEG, at optimum conditions of PEGylation was compared. It was found that succinimidyl carbonate at its optimum condition (pH=8.7, temperature =14 ° C and reaction time =60 min) is more effective than cyanuric chloride at its optimum condition (pH=8.7, temperature =14 ° C and reaction time =30 min) for RBC coating with mPEG.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF HTM / HTML
Volume Number	27
Alternate Webpage(s)	http://www.ijcce.ac.ir/pdf_6923_195a6340a2016640d1810451c86a8c01.html
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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