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Expression of various multidrug resistance-associated protein (MRP) homologues in brain microvessel endothelial cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Yan Han, Huaiyun Elmquist, William F. Miller, Donald W. |
| Copyright Year | 2000 |
| Abstract | Multidrug resistance-associated protein (MRP) actively transports a broad range of anionic compounds out of the cell. To date, six different homologues of MRP (i.e. MRP1-MRP6) have been identified. The current study examines the expression of the various MRP homologues in both primary cultured bovine brain microvessel endothelial cells (BBMEC) and the capillary-enriched fraction from bovine brain homogenates. RT-PCR analysis demonstrated the presence of MRP1, MRP4, MRP5 and MRP6 in both BBMEC and the capillary-enriched fractions of brain homogenates. While low levels of MRP3 were detected in the BBMEC, it was not observed in the capillary-enriched fraction. In addition, RT-PCR and Western blot studies indicated an absence of MRP2 expression in both blood-brain barrier preparations. The presence of several different MRP homologues in the brain microvessel endothelial cells may be important in controlling the permeability of the blood-brain barrier to organic anions. |
| Starting Page | 148 |
| Ending Page | 153 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0006-8993(00)02628-7 |
| PubMed reference number | 10973603 |
| Journal | Medline |
| Volume Number | 876 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0006899300026287 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0006899300026287?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/S0006-8993%2800%2902628-7 |
| Journal | Brain Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
