NDLI: High-throughput RNAi in Caenorhabditis elegans: genome-wide screens and functional genomics.

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High-throughput RNAi in Caenorhabditis elegans: genome-wide screens and functional genomics.

Content Provider	Semantic Scholar
Author	Sugimoto, Asako
Copyright Year	2004
Abstract	The phenomenon of RNA-mediated interference (RNAi) was first discovered in the nematode Caenorhabditis elegans, in which introduction of double-stranded RNA causes specific inactivation of genes with corresponding sequences. Technical advances in RNAi methodology and the availability of the complete genome sequence have enabled the high-throughput, genome-wide RNAi analysis of this organism. Several groups have used large-scale RNAi to systematically examine every C. elegans gene for knock-down phenotypes, providing basal information to be mined in more detailed studies. Now, in addition to functional genomic RNAi analyses, high-throughput RNAi is also routinely used for rapid, genome-wide screens for genes involved in specific biological processes. The integration of high-throughput RNAi experiments with other large-scale data, such as DNA microarrays and protein-protein interaction maps, enhances the speed and reliability of such screens. The accumulation of RNAi phenotype data dramatically accelerates our understanding of this organism at the genetic level.
Starting Page	81
Ending Page	91
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1111/j.1432-0436.2004.07202004.x
Alternate Webpage(s)	http://gen677.weebly.com/uploads/8/6/5/7/865764/sugimoto2004review.pdf
PubMed reference number	15066188
Alternate Webpage(s)	https://doi.org/10.1111/j.1432-0436.2004.07202004.x
Journal	Medline
Volume Number	72
Issue Number	2-3
Journal	Differentiation; research in biological diversity
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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