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Direct evidence of leukocyte adhesion in arterioles by angiotensin II.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Álvarez, Á. Carrillo Cerdá-Nicolás, Miguel Nabah, Yafa Naim Abu Mata, Manuel Issekutz, Andrew C. Panés, Julián Lobb, Roy R. Sanz, María Jesús Gil |
| Copyright Year | 2004 |
| Abstract | Although leukocytes adhere in arteries in various vascular diseases, to date no endogenous proinflammatory molecule has been identified to initiate leukocyte adhesion in the arterial vasculature. This study was undertaken to assess angiotensin II (Ang II)-induced leukocyte adhesion in arterioles in vivo. Rats received intraperitoneal injections of Ang II; 4 hours later, leukocyte recruitment in mesenteric microcirculation was examined using intravital microscopy. Ang II (1 nM) produced significant arteriolar leukocyte adhesion of mononuclear cells. Using function-blocking monoclonal antibodies (mAbs) against different rat cell adhesion molecules (CAMs), we discovered that this effect was dependent on P-selectin and beta(2)-integrin. In postcapillary venules, Ang II also induced leukocyte infiltration, which was reduced by P-selectin and by beta(2)- and alpha(4)-integrin blockade. Interestingly, neutrophils were the primary cells recruited in venules. Although beta(2)-integrin expression in peripheral leukocytes of Ang II-treated animals was not altered, it was increased in peritoneal cells. Immunohistochemical studies revealed increased P-selectin, E-selectin, intercellular cell adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) expression in response to Ang II in arterioles and venules. These findings provide the first evidence that Ang II causes leukocyte adhesion to the arterial endothelium in vivo at physiologically relevant doses. Therefore, Ang II may be a key molecule in cardiovascular diseases in which leukocyte adhesion to the arteries is a characteristic feature. |
| Starting Page | 98 |
| Ending Page | 104 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bloodjournal.org/content/bloodjournal/104/2/402.full.pdf?sso-checked=true |
| Alternate Webpage(s) | http://bloodjournal.hematologylibrary.org/content/104/2/402.full.pdf |
| PubMed reference number | 15044253v1 |
| Volume Number | 104 |
| Issue Number | 2 |
| Journal | Blood |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Angiotensin II Angiotensins Blood supply aspects Cardiovascular Diseases Cell Adhesion Molecules E-Selectin Endothelium of artery Leukocytes Mesentery Microcirculation Monoclonal Antibodies P-Selectin Selectins Structure of arteriole Structure of venule Vascular Cell Adhesion Molecule-1, human Vascular Diseases leukocyte cell-cell adhesion |
| Content Type | Text |
| Resource Type | Article |
