Antioxidant activity of CAPE (caffeic acid phenethyl ester) in vitro can protect human sperm deoxyribonucleic acid from oxidative damage.

Content Provider	Semantic Scholar
Author	Ayla, Şule Tunalı, Gülden Bilgiç, Bülent Emre Sofuoğlu, Kenan Özdemir, Armağan Tanrıverdi, Gamze Özdemir, Semra Soner, Burak Cem Öztürk, Bahar Karahüseyinoğlu, Serçin Aslan, Esra Seçkin, Ismail
Copyright Year	2018
Abstract	PURPOSE Sperm processing (e.g., centrifugation) used in preparation for assisted reproduction can result in excessive generation of reactive oxygen species (ROS) and potential sperm damage. The use of antioxidants during sperm processing has been shown to prevent iatrogenic sperm damage, including DNA damage. In this study, we evaluated the effect of caffeic acid phenethyl ester (CAPE) on oxidative stress mediated sperm dysfunction and DNA damage. METHODS Semen samples were obtained to liquefy at room temperature. After centrifugation and washing protocols, spermatozoa were incubated in a single step supplemented medium with either of 10, 50 or 100 μmol/L CAPE for 2 hours at 36 °C. After incubation period, MDA levels of seminal plasma were measured. The fragmentation in sperm DNA was detected by light microscopy via use of an aniline blue assay, while ultrastructural morphology was analyzed by transmission electron microscopy. RESULTS Significant increase has been observed in percent chromatin condensation (assessed by aniline blue staining) and Malondialdehyde (Mmol/L) in oligoasthenoteratozoospermia group before the centrifugation (0.57 ± 0.15). Incubation of samples with 100 μmol/L CAPE after centrifugation resulted in a significantly lower percent chromatin condensation compared to samples incubated without CAPE (0.42 ± 0.12) (P < 0.0033). Incubation of all samples with CAPE (10 μmol/L, 50 μmol/L, 100 μmol/L.) after centrifugation resulted in a significantly lower percentage of Malondialdehyde levels. CONCLUSIONS The data suggests that preincubation of spermatozoa with the antioxidant CAPE offers protection against oxidative DNA damage in vitro.
Starting Page	117
Ending Page	121
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1016/j.acthis.2018.01.001
PubMed reference number	29325972
Journal	Medline
Volume Number	120
Issue Number	2
Alternate Webpage(s)	http://www.posters2view.eu/eshre2015/data/043.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.acthis.2018.01.001
Journal	Acta histochemica
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article