Induktion und Reparatur von DNA-Doppelstrangbrüchen nach kombinierter Einwirkung von Cisplatin und Bestrahlung auf eukaryote Zellen

Content Provider	Semantic Scholar
Author	Wanke, Friederike
Copyright Year	2010
Abstract	The efficacy of treatment with a combination of radiotherapy and chemotherapy in the yeast Saccharomyces cerevisiae as suitable eukaryotic model is demonstrated by following the induction and repair of DNA double-strand breaks. These may be induced by ionizing radiation. Furthermore, the chemotherapeutic agent cisplatin may induce DNA double-strand breaks through cellular repair mechanisms. The aim of this study is to investigate the induction and repair of DNA double-strand breaks after a combination of treatment with cisplatin and radiation versus radiation alone under hypoxic conditions.The number of induced double-strand breaks caused by radiation under hypoxic conditions is dependent on dose. Following combined treatment with cisplatin and radiation, the radiation-induced double-strand breaks simply add to those induced by cisplatin. We identified no difference in the repair kinetics of double-strand breaks following radiation between cells treated with cisplatin and those not treated with cisplatin. We also detected no sensitizing effect of the treatment with cisplatin on radiation under hypoxic conditions.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://ediss.uni-goettingen.de/bitstream/handle/11858/00-1735-0000-0006-AFAE-B/wanke.pdf?sequence=1
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article