NDLI: Simultaneous extraction of carotenoids and transfructosylating enzyme from Xanthophyllomyces dendrorhous by a bead beater

Content Provider	Semantic Scholar
Author	Lee, Shu-Chih Fang, Tony J.
Copyright Year	2010
Abstract	Purpose of workConditions for the cell disruption of Xanthophyllomyces dendrorhous by using a bead beater have been optimized with respect to maximizing the recovery of carotenoids and transfructosylating enzymes in a one-step process.The optimal conditions for a one-step extraction of carotenoids and transfructosylating enzymes from Xanthophyllomyces dendrorhous were: 30 g dry cells/l being disintegrated with 0.5 mm diam. glass beads at 10°C for 15 min using a bead beater. More than 90% yeast disruption and, when extracted with hexane/ethyl acetate, 28.6 mg carotenoids/l in the organic solvent phase and 440 U transfructosylating enzyme activity/mg protein in the water phase were obtained.
Starting Page	109
Ending Page	112
Page Count	4
File Format	PDF HTM / HTML
DOI	10.1007/s10529-010-0400-z
PubMed reference number	20865441
Journal	Medline
Volume Number	33
Alternate Webpage(s)	http://benz.nchu.edu.tw/~tjfang/pdf/2011/Lee2011.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s10529-010-0400-z
Journal	Biotechnology Letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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