Ca2+ channel currents in type I carotid body cells of normoxic and chronically hypoxic neonatal rats

Content Provider	Semantic Scholar
Author	Peers, Chris Carpenter, Elisabeth Hatton, Christopher J. Wyatt, Christopher N. Bee, David E.
Copyright Year	1996
Abstract	Whole-cell patch-clamp recordings were used to study voltage-gated Ca2+ channel currents in type I carotid body cells of young rats born and reared in normoxia or in a chronically hypoxic (CH) environment (10% O2). Currents activated at potentials of -40 mV and more positive, and typically peaked at 0 mV in both groups of cells. Steady-state inactivation curves were similar in the two populations. Ca2+ currents were significantly larger in CH type I cells, but this was accounted for by the increased size of CH cells: current density was similar in both cell types. Nifedipine (5 microM) always partially inhibited currents and Bay K 8644 (2-5 microM) always enhanced currents, indicating the presence of L-type channels. In a small number of cells from each group, the N-type channel blocker omega-conotoxin GVIA caused partial, irreversible inhibition, but in most cells was without discernible effect. These results indicate that type I cells possess L-type Ca2+ channels, that N-type are expressed in some cells and that non-L, non-N-type channels are also present. Furthermore, chronic hypoxia does not appear to cause specific adaptive changes in the properties of Ca2+ channels in type I cells.
Starting Page	251
Ending Page	257
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1016/S0006-8993(96)00832-3
PubMed reference number	8955945
Journal	Medline
Volume Number	739
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0006899396008323
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0006899396008323?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/S0006-8993%2896%2900832-3
Journal	Brain Research
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article