NDLI: Recombinant human transforming growth factor-alpha stimulates the formation of osteoclast-like cells in long-term human marrow cultures.

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Recombinant human transforming growth factor-alpha stimulates the formation of osteoclast-like cells in long-term human marrow cultures.

Content Provider	Semantic Scholar
Author	Takahashi, Naoyuki Macdonald, B. R. Hon, Jeremy K. Winkler, Marjorie E. Derynck, Rik Mundy, Gregory R. Roodman, Garson David
Copyright Year	1986
Abstract	Transforming growth factor-alpha (TGF-alpha) is synthesized by a variety of tumor cell lines and stimulates osteoclastic bone resorption in vitro. The mechanism by which TGF-alpha increases osteoclast activity is unknown. We used a human marrow culture system that forms osteoclast-like multinucleated cells (MNCs) to determine the effects of recombinant human TGF-alpha on MNC formation. Addition of 0.01 ng/ml TGF-alpha for the 1st week followed by 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] for the subsequent 2 wk significantly increased MNCs. Treatment of these cultures with TGF-alpha without later addition of 1,25(OH)2D3 did not increase MNC formation. Autoradiographic studies revealed that TGF-alpha stimulated proliferation of precursors for MNCs, and 1,25(OH)2D3 increased their rate of fusion into MNCs. Addition of murine epidermal growth factor (EGF) (0.1 ng/ml) followed by 1,25(OH)2D3 also significantly stimulated MNC formation. These data suggest that TGF-alpha and EGF may stimulate bone resorption by increasing the proliferation of osteoclast precursors, which leads to increased numbers of osteoclasts.
File Format	PDF HTM / HTML
DOI	10.1172/JCI112677
PubMed reference number	3489738
Journal	Medline
Volume Number	78
Issue Number	4
Alternate Webpage(s)	https://dm5migu4zj3pb.cloudfront.net/manuscripts/112000/112677/JCI86112677.pdf
Alternate Webpage(s)	https://doi.org/10.1172/JCI112677
Journal	The Journal of clinical investigation
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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