NDLI: Overcoming the Gas–Liquid Mass Transfer of Oxygen by Coupling Photosynthetic Water Oxidation with Biocatalytic Oxyfunctionalization

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Overcoming the Gas–Liquid Mass Transfer of Oxygen by Coupling Photosynthetic Water Oxidation with Biocatalytic Oxyfunctionalization

Content Provider	Semantic Scholar
Author	Hoschek, Anna Bühler, Bruno Schmid, Andreas
Copyright Year	2017
Abstract	Gas-liquid mass transfer of gaseous reactants is a major limitation for high space-time yields, especially for O2 -dependent (bio)catalytic reactions in aqueous solutions. Herein, oxygenic photosynthesis was used for homogeneous O2 supply via in situ generation in the liquid phase to overcome this limitation. The phototrophic cyanobacterium Synechocystis sp. PCC6803 was engineered to synthesize the alkane monooxygenase AlkBGT from Pseudomonas putida GPo1. With light, but without external addition of O2 , the chemo- and regioselective hydroxylation of nonanoic acid methyl ester to ω-hydroxynonanoic acid methyl ester was driven by O2 generated through photosynthetic water oxidation. Photosynthesis also delivered the necessary reduction equivalents to regenerate the Fe2+ center in AlkB for oxygen transfer to the terminal methyl group. The in situ coupling of oxygenic photosynthesis to O2 -transferring enzymes now enables the design of fast hydrocarbon oxyfunctionalization reactions.
Starting Page	15146
Ending Page	15149
Page Count	4
File Format	PDF HTM / HTML
PubMed reference number	28945948v1
Alternate Webpage(s)	https://doi.org/10.1002/anie.201706886
DOI	10.1002/anie.201706886
Journal	Angewandte Chemie
Volume Number	56
Language	English
Access Restriction	Open
Subject Keyword	Alkanes Equivalent Weight Esters Ferrous Hydrocarbons Hydroxylation Methyl Group Mixed Function Oxygenases Photosynthesis Solutions Synechocystis Thioctic Acid Water 1000 MG/ML Injectable Solution negative regulation of alkane biosynthetic process oxidation oxygen evolving activity pelargonic acid
Content Type	Text
Resource Type	Article

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