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Effective anion exchange chromatographic purification of hepatopancreatic alkaline phosphatase of Red shrimp, Solenocera choprai
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nooralabettu, Krishna Prasad |
| Copyright Year | 2014 |
| Abstract | Summary Hepatopancreas of Red shrimp was homogenised at 3,000 rpm for 10 min at 4℃, clarified at RCF of 1681.1 × g for 5 min at 4℃, and concentrated at 65% ammonium sulfate saturation level at 0℃ to isolated alkaline phosphatase. Parameters of chromatographic purification of the alkaline phosphatase was optimised in DEAE-cellulose columns using binding buffer of pH 7.6, 8.0, 8.4, or 8.8 and ionic strength of 0.00, 0.05, 0.10, or 0.15 M NaCl, and elution buffers of gradient 0.10-0.25, 0.10-0.35, 0.10-0.45 or 0.10-0.55 M NaCl in 25 min and flow rate of 0.5, 1.0, 1.5, or 2.0 mL/min. Suitable binding buffer is of pH 8.4 and ionic strength 0.1 M NaCl at flow rate of 1 mL/min and effective elution buffer is of ionic gradient of 0.10-0.35 M NaCl in 25 min at a flow rate of 1.5 mL/min. |
| Starting Page | 41 |
| Ending Page | 51 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| Volume Number | 2 |
| Alternate Webpage(s) | http://plaza.umin.ac.jp/~e-jabs/2/2.41.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
