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MiR-7 involves the activation of Nrf 2 pathway by targeting Keap 1 in epileptic seizure
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yu, Liang Sun, Hongbin He, Baomin Li, Supin Ma, Shuai Yang, Lili Zhou, Dong |
| Copyright Year | 2016 |
| Abstract | MicroRNAs (miRNAs) are increasingly recognized as diagnostic biomarkers of epileptogenesis as well as targets to prevent or disrupt epilepsy. Keap1, a cytoplasmic inhibitory protein called Kelch-like ECH-associated protein 1, is also reported associated with epilepsy. However, the possible involvement of miRNAs in Keap1-mediated molecular basis for protection from epileptic seizure-induced brain damage is less understood. In the present study, Wistar rats were rapidly kindled in the amygdala. We found a dramatic upregulation of miR-7 and downregulation of Keap1 in the hippocampus of kindled rats, compared with control and sham group. Moreover, luciferase reporter gene assays identified that miR-7 was able to target 3’-untranslated region (3’-UTR) of Keap1 mRNA. To investigate the role of miR-7 in the protection of epilepsy mediated by Keap1/Nrf2 pathway, we used a transfection approach to overexpress miR-7, and then detected a consequential decrease in Keap1 mRNA and protein which subsequently results in an increased Nrf2 expression and cytoprotective enzymes (HO-1 and NQO1) expression. These results indicate that miR-7 may involve the protection of protection through targeting Keap1 and the subsequent activation of Nrf2 pathway. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ijcep.com/files/ijcep0018996.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
