NDLI: Simultaneous Estimation of Nebivolol Hydrochloride and Hydrochlorothiazide in Tablets by TLC-Densitometry

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Simultaneous Estimation of Nebivolol Hydrochloride and Hydrochlorothiazide in Tablets by TLC-Densitometry

Content Provider	Semantic Scholar
Author	Ahir, Keyur B. Patelia, Emanual M. Mehta, Falgun A.
Copyright Year	2012
Abstract	A simple, rapid and accurate thin-layer chromatography (TLC)–densitometric method has been established and validated for the simultaneous determination of nebivolol hydrochloride and hydrochlorothiazide in tablets. The method is based on TLC separation of the two drugs followed by densitometric measurements of their spots at 281 nm. The separation was carried out on Merck TLC aluminium plate of silica gel 60F 254 using toluene:ethyl acetate:methanol:ammonia (3:2.7:1.7:0.1 v/v/v) as a mobile phase. Nebivolol hydrochloride and hydrochlorothiazide gave sharp and well defined peak at Rf 0.68 and 0.38, respectively. Calibration curves for nebivolol hydrochloride and hydrochlorothiazide were linear in range 500-3000 ng/spot and 1000-6000 ng/spot, respectively. Method was successively applied to tablet formulation. No chromatographic interferences from the tablet excipients were found. The method was validated in accordance with the requirements of ICH guidelines.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.omicsonline.org/simultaneous-estimation-of-nebivolol-hydrochloride-and-hydrochlorothiazide-in-tablets-by-tlc-densitometry-2157-7064.1000141.pdf
Language	English
Access Restriction	Open
Subject Keyword	Aluminum Ammonia Densitometry Eluent Emoticon Exanthema Hydrochlorothiazide Merck Index Methanol Multi-level cell NG-PON2 One Thousand Pharmaceutical Excipient Requirement Silica Gel Silicon Dioxide Tablet Dosage Form Tablet computer Thin Layer Chromatography Toluene Volume fraction ethyl acetate nebivolol hydrochloride
Content Type	Text
Resource Type	Article

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