Evaluación de la utilidad clínica de la prueba ELISA de detección de Ag manano de Candida para el diagnóstico de la candidiasis invasiva en pacientes pediátricos

Content Provider	Semantic Scholar
Author	Martínez-Garnica, Diana Evelyn Jiménez-Jiménez, Marcela Ramírez-Guerrero, Celedonio López-Martínez, Briceida
Copyright Year	2015
Abstract	Context: Invasive candidiasis (IC) is the fourth leading cause of nosocomial bloodstream infections and associated mortality is greater than 40%. Diagnosis based on an immunoenzymatic technique, allows detection at an appropriate time, the mannan antigen circulating in the serum of patients. Objective: To determine the clinical utility of immunosorbent assay detection of mannan antigen in human serum in pediatric patients. Material and methods: Analytical parameters of the ELISA test (Candida PlateliaTM AG®) were evaluated: sensitivity, specifi city, PPV, NPV, compared to the gold standard which is the blood culture. Results: 538 serum samples from 348 patients were enrolled with sepsis. A who underwent screening mannan Ag. 197 samples (36.6%) were positive for mannan Ag, for you, only 61 samples (11.3%) had positive mycological culture and only 19 (3.5%) blood culture positive for yeast. The sensitivity and specifi city of the test were 86.4% and 65.5% respectively, the PPV was 9.6% and NPV 99.1%. Conclusions: In the diagnosis of IC, the result of the test for mannan Ag should not be taken as a data point, it is essential to have clinical, mycological cultures and blood cultures. Because of the high negative predictive value of the test (99.1%) consider that this test would help rule out a probable CI.
Starting Page	81
Ending Page	85
Page Count	5
File Format	PDF HTM / HTML
Volume Number	62
Alternate Webpage(s)	http://www.medigraphic.com/pdfs/patol/pt-2015/pt152b.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article