Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (pangasius sutchi) skin

Content Provider	Semantic Scholar
Author	Mahmoodani, Fatemeh Ardekani, Venus Sanaei Fern, See Siau Yusop, Salma Mohamad Babji, Abd. Salam
Copyright Year	2014
Abstract	In order to optimize the extraction of gelatin from pangasius catfish skin, a response surface method (RSM) involving a Central Composite Design (CCD) was applied. Four variables, namely NaOH concentration (0-0.3 N), acetic acid concentration (0.025-0.125 N), extraction time (2-4 h) and extraction temperature (40-80°C) were selected as independent variables for the optimization using RSM. The dependent variable was calculated by hydroxyproline recovery. The optimum conditions for extraction were produced by a pre-treatment of 0.2 N NaOH and 0.1 N acetic acid along with hot water extraction at 63.7°C for 2.41 h. The results showed that the predicted response by RSM (68.53%) closely matched the experimental response of 68.16%. The results indicated that the extracted gelatin possessed high gel strength (438 g) and high content of imino acid (proline and hydroxyproline) (18.01%) with a viscosity of 4.67 mPa s. The results showed that RSM was a great optimizing tool for extraction of gelatin from pangasius catfish skin. The gelatin was also proven to have significantly (p<0.05) higher quality of physicochemical properties than those from bovine skin gelatin.
Starting Page	995
Ending Page	1002
Page Count	8
File Format	PDF HTM / HTML
Volume Number	43
Alternate Webpage(s)	http://www.ukm.my/jsm/pdf_files/SM-PDF-43-7-2014/05%20Fatemeh.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article