NDLI: Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes

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Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes

Content Provider	Semantic Scholar
Author	Praetorius, Florian Dietz, Hendrik
Copyright Year	2017
Abstract	We describe an approach to bottom-up fabrication that allows integration of the functional diversity of proteins into designed three-dimensional structural frameworks. A set of custom staple proteins based on transcription activator–like effector proteins folds a double-stranded DNA template into a user-defined shape. Each staple protein is designed to recognize and closely link two distinct double-helical DNA sequences at separate positions on the template. We present design rules for constructing megadalton-scale DNA-protein hybrid shapes; introduce various structural motifs, such as custom curvature, corners, and vertices; and describe principles for creating multilayer DNA-protein objects with enhanced rigidity. We demonstrate self-assembly of our hybrid nanostructures in one-pot mixtures that include the genetic information for the designed proteins, the template DNA, RNA polymerase, ribosomes, and cofactors for transcription and translation.
File Format	PDF HTM / HTML
DOI	10.1126/science.aam5488
Alternate Webpage(s)	https://sd3a9d488837a8342.jimcontent.com/download/version/1493968950/module/9094458485/name/praetorius_science_2017_eaam5488.full.pdf
Alternate Webpage(s)	https://sd3a9d488837a8342.jimcontent.com/download/version/1493968953/module/9094458585/name/praetorius_science_SM_2017.pdf
PubMed reference number	28336611
Alternate Webpage(s)	https://doi.org/10.1126/science.aam5488
Journal	Medline
Volume Number	355
Journal	Science
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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