NDLI: Determination of Phorate and Its Metabolite Phorate Sulfone Residues in the Egg by High Performance Liquid Chromatography-tandem Mass Spectrometry

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Determination of Phorate and Its Metabolite Phorate Sulfone Residues in the Egg by High Performance Liquid Chromatography-tandem Mass Spectrometry

Content Provider	Semantic Scholar
Author	Guo, Hong-Bin Ye, Jianzhi Lin, Ling Yang, Chunliang
Copyright Year	2018
Abstract	In this study, we present a newly developed fast and sensitive method for the quantification of phorate and its metabolite phorate sulfone residues in the egg, using solid phase extraction and UPLC-MS/MS analysis.The eggs sample were extracted with acetonitrile under neutral conditions in this paper , cleaned up by the glass funnel plugged with cotton and salts equipped with anhydrous sodium sulfate and neutral alumina, and determinatied by waters high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). There are good linear correlations between the peak area and the corresponding concentration of phorate and its metabolite phorate sulfone in the range of 2.00-100.00 ng/mL and the linear coefficient of the three pesticides is above 0.9995. The recovery rate of phorate and its metabolite phorate sulfone ranged from 78.6% to 95.6%, and with the batch relative deviation ranged from 0.3% to 5.6%. The lower limit detection (LOD) of the phorate sulfone, phorate and phorate sulfoxide were 0.0005 mg/kg , 0.0005mg/kg and 0.0005 mg/kg, respectively. The phorate, phorate sulfone and phorate sulfoxide's quantitative(LOQ)were 0.0015 mg/kg, 0.0015 mg/kg and 0.0015 mg/kg, respectively. The results show that UPLC-MS/MS in conjunction with phorate and its metabolite phorate sulfone is a powerful tool for screening the inhibitory effects of eggs and can thus be used for safety control in food processing procedures.
File Format	PDF HTM / HTML
DOI	10.5220/0008185600630068
Alternate Webpage(s)	https://www.scitepress.org/Papers/2018/81856/81856.pdf
Alternate Webpage(s)	https://doi.org/10.5220/0008185600630068
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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