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The Gene Expression Status of the PI3K/AKT/mTOR Pathway in Gastric Cancer Tissues and Cell Lines
| Content Provider | Semantic Scholar |
|---|---|
| Author | Riquelme, Ismael Tapia, Oscar Espinoza, Jaime A. Leal, Pamela Buchegger, Kurt Sandoval, Alejandra Bizama, Carolina Araya, Juan Peek, Richard M. Roa, Juan Carlos |
| Copyright Year | 2016 |
| Abstract | The PI3K/AKT/mTOR pathway plays a crucial role in the regulation of multiple cellular functions including cell growth, proliferation, metabolism and angiogenesis. Emerging evidence has shown that deregulation of this pathway has a role promoting gastric cancer (GC). The aim was to assess the expression of genes involved in this pathway by qPCR in 23 tumor and 23 non-tumor gastric mucosa samples from advanced GC patients, and in AGS, MKN28 and MKN45 gastric cancer cell lines. Results showed a slight overexpression of PIK3CA, PIK3CB, AKT1, MTOR, RPS6KB1, EIF4EBP1 and EIF4E genes, and a slightly decreased PTEN and TSC1 expression. In AGS, MKN28 and MKN45 cells a significant gene overexpression of PIK3CA, PIK3CB, AKT1, MTOR, RPS6KB1 and EIF4E, and a significant repression of PTEN gene expression were observed. Immunoblotting showed that PI3K-β, AKT, p-AKT, PTEN, mTOR, p-mTOR, P70S6K1, p-P70S6K1, 4E-BP1, p-4E-BP1, eIF4E and p-eIF4E proteins were present in cell lines at different levels, confirming activation of this pathway in vitro. This is the first time this extensive panel of 9 genes within PI3K/AKT/mTOR pathway has been studied in GC to clarify the biological role of this pathway in GC and develop new strategies for this malignancy. |
| Starting Page | 797 |
| Ending Page | 805 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s12253-016-0066-5 |
| PubMed reference number | 27156070 |
| Journal | Medline |
| Volume Number | 22 |
| Alternate Webpage(s) | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5890336&blobtype=pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s12253-016-0066-5 |
| Journal | Pathology & Oncology Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
